cloning dna cdna synthesis kit (TaKaRa)
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TaKaRa
cloning dna cdna synthesis kit
Cloning Dna Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cloning+dna+cdna+synthesis+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/10__15586_slash_aei__v53i4__1380-15-25-32
Average 99 stars, based on 15291 article reviews
Cloning Dna Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cloning+dna+cdna+synthesis+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/10__15586_slash_aei__v53i4__1380-15-25-32
Average 99 stars, based on 15291 article reviews
cloning dna cdna synthesis kit - by Bioz Stars,
2026-11
99/100 stars
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RNA Extraction:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a Real-time Polymerase Chain Reaction:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a Reverse Transcription:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a Cloning:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a cDNA Synthesis:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a SYBR Green Assay:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a Polymerase Chain Reaction:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a Reverse Transcription Polymerase Chain Reaction:Article Title: DEPTOR alleviates LPS-induced inflammation and ER stress in WI-38 cells Article Snippet: To establish an inflammatory model, WI-38 cells were treated with LPS at a concentration of 10 μg/mL (ST1730; Beyotime Biotechnology, China) for 12 h. For gene overexpression studies, cells were transfected with a human DEPTOR overexpression plasmid (Addgene, MA, USA) or an empty vector control (pCDNA3.1) using LipofectamineTM 3000 transfection reagent (Cat# L3000008; Invitrogen, Carlsbad, CA, USA), following the manufacturer’s instructions. .. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted using RNAiso Plus reagent (Cat# 9109; Takara, Japan) and reverse-transcribed using a |